This website allows the exploration of CRISPR interference (CRISPRi) data on enhancers in normal human astrocytes (NHAs), from Green et al. 2024. For each enhancer-gene pair tested in the screen, the following data is available:
Statistical significance and effect size (Hit, log2FC, P, FDR), displayed in a searchable table and displayed on the corresponding Volcano plot:
- search the table by gene name or hg38 genome coordinates.
- to download functional annotation data for the enhancer or gene in the selected pair click on the Annot button. Information on the functional annotation data is available in the Annotation Readme.txt.
ATAC-seq, TT-seq, and Deep-learning-based functional variant annotation, displayed as a UCSC genome browser track collection and heatmap:
- select an enhancer-gene pair in the table OR plot and click the UCSC button to navigate to the enhancer position.
- select an enhancer-gene pair and click the prediction button to navigate to the enhancer's DeepSEA (beluga) heatmap viewer.
Full tables are also directly downloadable for Enhancer Annotations (Readme) and Gene Annotations (Readme).
CRISPRi screening of enhancers in human primary astrocytes